Zhongcheng Shi, Michael Hoang Nguyen, Yuan Yao, Shixia Huang. High-Throughput Post-Translational Modification Analyses by Reverse Phase Protein Array. Protein Arrays: methods and applications. 2024 ...
Cell or tissue lysates are arrayed on nitrocellulose-coated slides (Grace Bio-labs, Bend, OR) in triplicate using the Aushon 2470 Arrayer (Aushon BioSystems, Billerica, MA) (technical replicates).
Reverse phase protein array is a high-throughput technology that performs protein assays on thousands of samples simultaneously, including tissue and cell lysates, serum, plasma or other body fluids.
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